Identification and application of a novel deoxynivalenol-degrading enzyme from Youhaiella tibetensis

Deoxynivalenol (DON) poses a significant threat to human health due to its widespread distribution and biological toxicity. Here, we identified a novel DON-degrading enzyme from Youhaiella tibetensis (YoDDH). YoDDH exhibited the highest activity against DON at pH 4.5 and 40 ℃, in the presence of Ca2+ and the pyrroloquinoline quinone (PQQ). Additionally, YoDDH displayed remarkable thermostability at 40 ℃, with a half-life of 24 h and a Tm value of 48.5 ℃. Notably, phenazine methosulfate (PMS) and 2,6-dichlorophenolindophenol (DCPIP) can also serve as electron acceptors for YoDDH. After incubation in the optimal conditions for 3 h, YoDDH degraded 73 % of DON (100 μM) finally. The kcat and kcat /Km of YoDDH towards DON was determined as 1.65 s-1 and 1526 M-1·s-1 in the presence of PMS. The 3-keto-DON was verified as the degradation product. This identified YoDDH presents a promising candidate for DON decontamination in the food and feed industry.