Alpinia oxyphylla Fructus is one of the traditional Chinese medicine plants in the treatment of kidney injury.In clinical practice, crude Alpinia oxyphylla Fructus (CAOF) and salt-processed Alpinia oxyphylla Fructus (SAOF) are the two commonly used drugs specificated in the prevention and treatment of diabetic nephropathy (DN).However, the intestinal micro ecology regulation between CAOF and SAOF on DN has not been reported.In this paper, intestinal micro ecology regulation activities between CAOF and SAOF in DN rats were compared and analyzed by short-chain fatty acids (SCFAs) and intestinal flora analysis.The results showed that both SAOF and CAOF can regulate the intestinal flora metabolite SCFAs level in DN rats, reduce blood glucose concentration and improve inflammatory reaction.The intestinal flora analysis showed SAOF and CAOF could increase the intestinal bacterial diversity.The treatment of renal injury may be related to their increased intestinal bacterial diversity.