显微镜
堆积
分辨率(逻辑)
光学(聚焦)
表征(材料科学)
人工智能
光学镊子
投影(关系代数)
旋转(数学)
光学成像
图像分辨率
光学显微镜
材料科学
计算机科学
光学
模式识别(心理学)
计算机视觉
纳米技术
化学
物理
扫描电子显微镜
有机化学
算法
作者
Yaoran Liu,Rohit Unni,Xin Lou,Mingcheng Yang,Yuebing Zheng
出处
期刊:Nano Letters
[American Chemical Society]
日期:2023-05-30
卷期号:23 (11): 5148-5154
标识
DOI:10.1021/acs.nanolett.3c01077
摘要
Three-dimensional (3D) characterization of organisms is important for the study of cellular phenotypes, structural organization, and mechanotransduction. Existing optical techniques for 3D imaging rely on focus stacking or complex multiangle projection. Focus stacking has deleterious axial resolution due to the one-angle optical projection. Herein, we achieve high-resolution 3D imaging and classification of organisms based on standard optical microscopy coupled to optothermal rotation. Through a seamless fusion of optical trapping and rotation of organisms on a single platform, our technique is applicable to any organism suspended in clinical samples, enabling contact-free and biocompatible 3D imaging. Moreover, when applying deep learning to distinguish different types of biological cells with high similarity, we demonstrate that our platform improves the classification accuracy (96% vs 85%) while using one-tenth the number of training samples compared with conventional deep-learning-based classification.
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