生物
黄萎病
大丽花黄萎病
基因
巴巴多斯棉
棉属
基因组
黄萎病
基因家族
遗传学
倍性
系统发育树
植物抗病性
植物
作者
Shuhan Yang,Qún Gě,Sumei Wan,Zhihao Sun,Yu Chen,Yanfang Li,Qiankun Liu,Jǔwǔ Gōng,Xiànghuī Xiāo,Quánwěi Lú,Yùzhēn Shí,Renhai Peng,Hǎihóng Shāng,Guodong Chen,Péngtāo Li
出处
期刊:Genes
[MDPI AG]
日期:2023-02-13
卷期号:14 (2): 477-477
被引量:1
标识
DOI:10.3390/genes14020477
摘要
Polyphenol oxidases (PPOs) are copper-binding metalloproteinases encoded by nuclear genes, ubiquitously existing in the plastids of microorganisms, plants, and animals. As one of the important defense enzymes, PPOs have been reported to participate in the resistant processes that respond to diseases and insect pests in multiple plant species. However, PPO gene identification and characterization in cotton and their expression patterns under Verticillium wilt (VW) treatment have not been clearly studied. In this study, 7, 8, 14, and 16 PPO genes were separately identified from Gossypium arboreum, G. raimondii, G. hirsutum, and G. barbadense, respectively, which were distributed within 23 chromosomes, though mainly gathered in chromosome 6. The phylogenetic tree manifested that all the PPOs from four cotton species and 14 other plants were divided into seven groups, and the analyses of the conserved motifs and nucleotide sequences showed highly similar characteristics of the gene structure and domains in the cotton PPO genes. The dramatically expressed differences were observed among the different organs at various stages of growth and development or under the diverse stresses referred to in the published RNA-seq data. Quantitative real-time PCR (qRT-PCR) experiments were also performed on the GhPPO genes in the roots, stems, and leaves of VW-resistant MBI8255 and VW-susceptible CCRI36 infected with Verticillium dahliae V991, proving the strong correlation between PPO activity and VW resistance. A comprehensive analysis conducted on cotton PPO genes contributes to the screening of the candidate genes for subsequent biological function studies, which is also of great significance for the in-depth understanding of the molecular genetic basis of cotton resistance to VW.
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