Multifunctional injectable hydrogel incorporating EGCG-Cu complexes for synergistic antibacterial, immunomodulatory, and osteogenic therapy in periodontitis

牙周炎 明胶 牙龈卟啉单胞菌 生物相容性 再生(生物学) 抗菌剂 体内 化学 氧化应激 炎症 药理学 牙科 癌症研究 医学 免疫学 生物化学 细胞生物学 生物 生物技术 有机化学
作者
Yajuan Hu,Wei Xu,Linghan Sun,Xuemin Ma,Peirong Zhou,Chuankai Zhang,Rui Cai,Xia Wang,Hua Yang,Gang Tao,Junliang Chen,Yun He
出处
期刊:Materials today bio [Elsevier BV]
卷期号:32: 101907-101907 被引量:9
标识
DOI:10.1016/j.mtbio.2025.101907
摘要

Periodontitis is a chronic inflammatory disease characterized by oxidative stress, immune system imbalance, and the progressive destruction of periodontal tissues. Traditional treatment approaches are limited by the incomplete eradication of pathogens, the risk of antibiotic resistance, inadequate control of inflammation and oxidative stress, and restricted tissue regeneration capacity. Therefore, this study proposes an injectable multifunctional Laponite/gelatin hydrogel loaded with epigallocatechin gallate (EGCG)-copper ion (Cu 2+ ) complexes as a localized therapy for periodontitis. EGCG exhibits antioxidant, anti-inflammatory, and antimicrobial properties; however, its clinical application is hindered by poor stability and bioavailability. Cu 2+ coordination enhances EGCG stability and antioxidant capacity while improving its antimicrobial efficacy. Experimental results demonstrate that the Laponite/gelatin hydrogel is adaptable for the localized delivery of EGCG-Cu, with bioactive metal ions such as Li + , Mg 2+ , and Si 4+ contained in Laponite, which promote osteogenesis and periodontal tissue regeneration. In vitro and in vivo studies confirm that this hydrogel exhibits excellent biocompatibility, effectively inhibits Porphyromonas gingivalis , suppresses M1 polarization while promoting M2 polarization, and facilitates periodontal tissue repair. Therefore, this study provides promising insights into a localized therapeutic strategy for periodontitis. • Multiple ROS Scavenging Capacity: Cu 2 + catalyzes antioxidant reactions, boosting EGCG’s ability to scavenge reactive oxygen species (ROS), thereby more effectively alleviating oxidative stress. • Injectability and Adaptability: Its adaptability and self-healing properties enable it to fill moist and complex periodontal defects, providing a stable three-dimensional scaffold that promotes cell adhesion and tissue regeneration. • Immunomodulatory Performance: It effectively inhibits M1 polarization of macrophages and promotes their polarization toward the M2 phenotype. • Osteogenic Inductive Function: Cu 2+ and Laponite synergistically exert osteogenic induction, facilitating alveolar bone regeneration in the context of periodontal inflammation. • Biocompatibility: The Lap-Gel/H-E-Cu hydrogel demonstrates good biocompatibility in vitro, showing low toxicity to red blood cells, RAW264.7 cells, and BMSCs. • In Vitro and In Vivo Validation: Through in vitro experiments and animal models, the anti-inflammatory and osteogenic effects of the Lap-Gel/H-E-Cu hydrogel system were validated.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
一和发布了新的文献求助10
1秒前
整齐的凌兰应助孤独幻枫采纳,获得10
1秒前
科研通AI6.3应助孤独幻枫采纳,获得10
1秒前
shuai发布了新的文献求助10
2秒前
3秒前
DS关闭了DS文献求助
4秒前
sjfczyh发布了新的文献求助10
4秒前
5秒前
田様应助yiiqianzhang采纳,获得10
5秒前
7秒前
8秒前
科研通AI2S应助shuai采纳,获得10
9秒前
9秒前
9秒前
Shaylee发布了新的文献求助10
11秒前
SciGPT应助青岑采纳,获得10
11秒前
轻舟完成签到,获得积分10
11秒前
12秒前
杨lan完成签到,获得积分10
13秒前
13秒前
法号胡来完成签到,获得积分10
13秒前
yihoxu发布了新的文献求助10
13秒前
14秒前
14秒前
mm发布了新的文献求助10
15秒前
轻舟发布了新的文献求助10
16秒前
jianxin发布了新的文献求助10
16秒前
momokop完成签到,获得积分10
17秒前
手动阀完成签到 ,获得积分10
17秒前
Zz完成签到,获得积分10
17秒前
YRHM发布了新的文献求助150
18秒前
爆米花应助WGQ采纳,获得10
19秒前
everything发布了新的文献求助10
19秒前
Tohka发布了新的文献求助10
19秒前
19秒前
杨lan发布了新的文献求助10
19秒前
Shaylee完成签到,获得积分10
21秒前
林天完成签到,获得积分10
21秒前
生动的向日葵完成签到,获得积分10
21秒前
22秒前
高分求助中
Malcolm Fraser : a biography 700
Signals, Systems, and Signal Processing 610
天津市智库成果选编 600
Climate change and sports: Statistics report on climate change and sports 500
Forced degradation and stability indicating LC method for Letrozole: A stress testing guide 500
Organic Reactions Volume 118 400
A Foreign Missionary on the Long March: The Unpublished Memoirs of Arnolis Hayman of the China Inland Mission 400
热门求助领域 (近24小时)
化学 材料科学 医学 生物 纳米技术 工程类 有机化学 化学工程 生物化学 计算机科学 物理 内科学 复合材料 催化作用 物理化学 光电子学 电极 细胞生物学 基因 无机化学
热门帖子
关注 科研通微信公众号,转发送积分 6466511
求助须知:如何正确求助?哪些是违规求助? 8273005
关于积分的说明 17639479
捐赠科研通 5541257
什么是DOI,文献DOI怎么找? 2907964
邀请新用户注册赠送积分活动 1884937
关于科研通互助平台的介绍 1732988