免疫球蛋白轻链
血液疗法
血液学
医学
血清蛋白电泳
骨髓
单克隆
医学实验室
内科学
病理
抗体
单克隆抗体
免疫学
作者
Maria Cruz Cárdenas,Ramón García‐Sanz,Noemí Puig,David Pérez-Surribas,Juan Flores‐Montero,María Ortiz-Espejo,Javier de la Rubia,Elena Cruz-Iglesias
标识
DOI:10.1515/cclm-2023-0326
摘要
Monoclonal gammopathies (MG) are characterized by the proliferation of plasma cells that produce identical abnormal immunoglobulins (intact or some of their subunits). This abnormal immunoglobulin component is called monoclonal protein (M-protein), and is considered a biomarker of proliferative activity. The identification, characterization and measurement of M-protein is essential for the management of MG. We conducted a systematic review of the different tests and measurement methods used in the clinical laboratory for the study of M-protein in serum and urine, the biochemistry and hematology tests necessary for clinical evaluation, and studies in bone marrow, peripheral blood and other tissues. This review included literature published between 2009 and 2022. The paper discusses the main methodological characteristics and limitations, as well as the purpose and clinical value of the different tests used in the diagnosis, prognosis, monitoring and assessment of treatment response in MG. Included are methods for the study of M-protein, namely electrophoresis, measurement of immunoglobulin levels, serum free light chains, immunoglobulin heavy chain/light chain pairs, and mass spectrometry, and for the bone marrow examination, morphological analysis, cytogenetics, molecular techniques, and multiparameter flow cytometry.
科研通智能强力驱动
Strongly Powered by AbleSci AI