免疫分析
铝箔
基质(水族馆)
检出限
箔法
选择性
材料科学
表面增强拉曼光谱
胶体金
拉曼光谱
纳米技术
色谱法
化学
纳米颗粒
抗体
光学
复合材料
免疫学
医学
拉曼散射
生物化学
图层(电子)
物理
地质学
催化作用
海洋学
作者
Rostislav Bukasov,Alisher Sultangaziyev,Zhanar Kunushpayeva,Alisher Rapikov,Dina Dossym
摘要
The first application of aluminum foil (Al F) as a low-cost/high-availability substrate for sandwich immunoassay using surface-enhanced Raman spectroscopy (SERS) is reported. Untreated and unmodified Al F and gold film are used as substrates for sandwich SERS immunoassay to detect tuberculosis biomarker MPT64 and human immunoglobulin (hIgG) in less than 24 h. The limits of detection (LODs) for tuberculosis (TB) biomarker MPT64 on Al foil, obtained with commercial antibodies, are about 1.8-1.9 ng/mL, which is comparable to the best LOD (2.1 ng/mL) reported in the literature for sandwich ELISA, made with fresh in-house antibodies. Not only is Al foil competitive with traditional SERS substrate gold for the sandwich SERS immunoassay in terms of LOD, which is in the range 18-30 pM or less than 1 pmol of human IgG, but it also has a large cost/availability advantage over gold film. Moreover, human IgG assays on Al foil and Si showed better selectivity (by about 30-70% on Al foil and at least eightfold on Si) and a nonspecific response to rat or rabbit IgG, in comparison to the selectivity in assays using gold film.
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