MoS2/PDA@Cu composite as a peroxidase-mimicking enzyme with high-effect antibacterial and anticancer activity

Since nanozymes were proposed, their applications have become more and more extensive. As a research hotspot in recent years, MoS₂ also shows many enzyme-like properties. However, as a novel peroxidase, MoS₂ has the disadvantage of a low maximum reaction rate. In this study, the MoS₂/PDA@Cu nanozyme was synthesized by a wet chemical method. The modification of PDA on the surface of MoS₂ achieved the uniform growth of small-sized Cu Nps. The obtained MoS₂/PDA@Cu nanozyme displayed excellent peroxidase-like activity and antibacterial properties. The minimum inhibitory concentration (MIC) of the MoS₂/PDA@Cu nanozyme against S. aureus reached 25 μg mL^-1. Furthermore, it showed a more pronounced inhibitory effect on bacterial growth with the addition of H₂O₂. The maximum reaction rate (V_max) of the MoS₂/PDA@Cu nanozyme is 29.33 × 10^-8 M s^-1, which is significantly higher as compared to that of HRP. It also exhibited excellent biocompatibility, hemocompatibility and potential anticancer properties. When the concentration of the nanozyme was 160 μg mL^-1, the viabilities of 4T1 cells and Hep G2 cells were 45.07% and 32.35%, respectively. This work indicates that surface regulation and electronic transmission control are good strategies for improving peroxidase-like activity.