适体
癌胚抗原
表面增强拉曼光谱
纳米棒
线性范围
化学
纳米技术
拉曼光谱
检出限
色谱法
材料科学
癌症
分子生物学
拉曼散射
光学
医学
物理
内科学
生物
作者
Rui Li,Lijun Li,Yan Zhang,Xin Lin,Heyuanxi Guo,Chubing Lin,Jun Feng
标识
DOI:10.1177/00037028221131577
摘要
Carcinoembryonic antigen (CEA) is a cancer-related tumor marker, which is commonly used for preventive screening, auxiliary diagnosis, and recurrence monitoring. Therefore, it is of great significance to develop a new CEA detection method. In this paper, we developed an SERS aptasensor for CEA based on silver nanorod array chip, thiol aptamer, and 4-mercaptophenylboronic acid (4-MPBA). The silver nanorod array chip modified by CEA thiol aptamer (aptamer-SH) was used as SERS capture substrates. Ag@4-MPBA was used as a surface-enhanced Raman spectroscopy (SERS) tag. This proposed SERS aptasensor could detect CEA down to 0.447 pg·mL −1 with a wide linear range from 1 pg·mL −1 to 100 ng·mL −1 (R 2 = 0.9907). The recovery of the standard addition test for CEA in serum was between 97.25% and 102.67%, and the RSD ≤ 2.52% ( n = 3). The sensor has the advantages of good specificity, high sensitivity, and a wide linear range. It provides a new method for the detection of CEA in serum.
科研通智能强力驱动
Strongly Powered by AbleSci AI