细胞外基质
去细胞化
再生(生物学)
脚手架
超细纤维
纳米纤维
材料科学
静电纺丝
生物医学工程
体内
组织工程
血管平滑肌
聚合物
纳米技术
化学
细胞生物学
复合材料
平滑肌
生物化学
医学
生物
生物技术
内科学
作者
Siyang Liu,Lei Yao,Yumeng Wang,Yang Li,Yanju Jia,Yueyue Yang,Na Li,Y. Hu,Deling Kong,Xianhao Dong,Kai Wang,Meifeng Zhu
标识
DOI:10.1016/j.bioactmat.2022.08.018
摘要
The inertness of synthetic polymer materials and the insufficient mechanical strength of reprocessed decellularized extracellular matrix (dECM) limited their promotive efforts on tissue regeneration. Here, we prepared a hybrid scaffold composed of PCL microfibers and human placental extracellular matrix (pECM) nanofibers by co-electrospinning, which was grafted with heparin and further absorbed with IL-4. The hybrid scaffold with improved hemocompatibility firstly switched macrophages to anti-inflammatory phenotype (increased by 18.1%) and then promoted migration, NO production, tube formation of endothelial cells (ECs), and migration and maturation of vascular smooth muscle cells (VSMCs), and ECM deposition in vitro and in vivo. ECs coverage rate increased by 8.6% and the thickness of the smooth muscle layer was 1.8 times more than PCL grafts at 12 wks. Our study realized the complementary advantages of synthetic polymer materials and dECM materials, and opened intriguing perspectives for the design and construction of small-diameter vascular grafts (SDVGs) and immune-regulated materials for other tissue regeneration.
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