化学
抗坏血酸
检出限
荧光
免疫分析
赭曲霉毒素A
铈
色谱法
基质(水族馆)
核化学
无机化学
真菌毒素
地质学
免疫学
抗体
物理
海洋学
生物
量子力学
食品科学
作者
Wang Chen,Xingping Zhang,Qian-Chong Zhang,Guohao Zhang,Shixiang Wu,Hualin Yang,Yu Zhou
标识
DOI:10.1016/j.aca.2022.340445
摘要
Ochratoxin A (OTA) is one of the most prevalent and toxic mycotoxins. Ultrasensitive and convenient detection of OTA is urgent demanded for public health. In this work, a dual-readout immunoassay was established for the detection of OTA based on Ce4+ oxidizing 3,3',5,5'-tetramethylbenzidine (TMB) and Ce3+ inducing aggregation induced emission (AIE) of Au nanoclusters (AuNCs). Under alkaline phosphatase (ALP), the ascorbic acid 2-phosphate (AA2P) can form ascorbic acid (AA) by dephosphorylation. The AA can reduce Ce4+ to generate Ce3+, which induced the AIE of AuNCs to enhance the fluorescence intensity of AuNCs. Meanwhile, unreacted Ce4+ oxidized TMB to form blue oxTMB. Thus, a dual-readout immunoassay was developed based on AIE of AuNCs and TMB as substrate. The limits of detection (LODs) were as low as 0.62 ng/mL for fluorescent assay and 0.81 ng/mL for colorimetric assay. The recoveries of OTA from corn were 94.4%-107.7% for the fluorescent mode and 93.7%-106.9% for the colorimetric mode. The results verified that the cerium ions triggered dual-readout immunoassay was reliable to sensitive detect OTA in corn samples.
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