化学
核酸
肽
生物化学
肽核酸
DNA
核糖核酸
寡核苷酸
贮藏蛋白
核酸酶
氨基酸
食品科学
作者
Sudarat Khadsai,Noppadol Seeja,Metha Rutnakornpituk,Tirayut Vilaivan,Maliwan Nakkuntod,Waroon Suwankitti,Filip Kielar,Boonjira Rutnakornpituk
标识
DOI:10.1016/j.foodchem.2020.127812
摘要
Abstract Here, we describe DNA enrichment of the zein gene from maize using pyrrolidinyl peptide nucleic acid (PNA) immobilized on a magnetic solid support as a capture element. Magnetite nanoparticles (MNP) with a capacity of 373 pmol PNA/mg and coated with poly(N-acryloylglycine) (PNAG) showed a good response to magnetic field. The PNA probe immobilized on the MNP discriminated between non-complementary and complementary DNA using fluorophore-tagged DNA as a model. We applied this system for the enrichment of the zein gene from maize in eight cereal product samples. After DNA desorption from the MNP, and its amplification via polymerase chain reaction (PCR), gel electrophoresis indicated that only cereal samples containing the zein gene from maize yielded positive results, indicating a high binding specificity between the PNA used and the complementary DNA. This PNA-functionalized MNP is potentially useful as an effective nano-solid support for DNA enrichment from other samples.
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