Biological activity of Brassica rapa L. polysaccharides on RAW264.7 macrophages and on tumor cells

Abstract Polysaccharides are a type of natural macromolecule widely existing in nature, and its pharmacological activity has attracted wide research attention. In this study, Brassica rapa L. polysaccharides were taken as the research object, and a preliminary study of the immune activity and mechanism of the antitumor activity of these polysaccharides in vitro was carried out. Five polysaccharides, namely, BRP, BRNP-1, BRNP-2, BRAP-1, and BRAP-2, were compared in terms of their ability to inhibit the growth of three types of cancer cells, namely, A549, AGS, and HepG2. The most effective polysaccharides were screened out, and their mechanism was studied. Immunoassay results showed that the five polysaccharides not only promoted the growth of RAW264.7 cells but also stimulated their endocytic/pinocytosis activity and released NO, TNF, IL-6 cytokines, especially BRP. In vitro antitumor experiments showed that BRP has a significant inhibitory effect (*P