Miniaturized Electrochemical Device for In-Situ Monitoring of Glucose, Lactate, Dissolved Oxygen, PH, and Temperature in Yeast Culture

This paper presents the integration on a single chip of multiple electrochemical sensors based on amperometric and potentiometric techniques, as well as of a physical sensor. The prototype comprises six sensors for in-situ monitoring of important parameters of a bioprocess; glucose, lactate, pH, cell density, dissolved oxygen, and temperature. All sensor elements have been calibrated and showed acceptable detection performance. We also demonstrate a preliminary experiment for in-situ monitoring in a yeast culture to better understand the response of the proposed microsensors towards yeast fermentations application.