An Electrochemical Aptamer Biosensor for Bisphenol A on a Carbon Nanofibre‐silver Nanoparticle Immobilisation Platform

Abstract A nanocomposite platform of silver nanoparticles and carbon nanofibres (AgCNFs) was used to immobilise a bisphenol A specific 63‐mer ssDNA aptamer to form a biosensor. The fabrication process of the biosensor was studied with electrochemical impedance spectroscopy and cyclic voltammetry in the presence of [Fe(CN) 6 ] 3−/4− as redox probe. The biosensor detected bisphenol A in a linear range of 0.1–10 nM, with a limit of detection of 0.39 nM using square wave voltammetry (SWV). The biosensor exhibited good selectivity in the presence of interfering species at 100‐fold concentrations and was used to detect BPA in real water sample.