The expression of tubulin polymerization promoting protein TPPP/p25α is developmentally regulated in cultured rat brain oligodendrocytes and affected by proteolytic stress

Abstract The tubulin polymerization‐promoting protein (TPPP)/p25α was identified as a brain specific protein, is associated with microtubules (MTs) in vitro and can promote abnormal MT assembly. Furthermore it has aggregation promoting properties and is a constituent in pathological protein deposits of neurodegenerative diseases. In the brain, TPPP/p25α is present in myelinating oligodendrocytes. Here we show, using cultured rat brain oligodendrocytes, that TPPP/p25α expression is increasing during development in culture, and particularly in immature cells is associated with the centrosome. MT binding properties in oligodendrocytes are rather low, however, when MTs are disassembled by nocodazole, TPPP/p25α accumulates in the perinuclear region. Treatment of oligodendrocytes with the proteasomal inhibitor MG‐132 (1 μM; 18 h) caused an increase in the amount of TPPP/p25α by about 40%, a decrease in its solubility, and led to the appearance of TPPP/p25α‐positive cytoplasmic inclusions, which stained with thioflavin S and resembled inclusion bodies. Hence, it might be speculated that acute or chronic malfunction of the proteasomal degradation system, leading to the accumulation of aggregation prone proteins and the pro‐aggregatory protein TPPP/p25α or to the aggregation of TPPP/p25α on its own, is causally related to the protein aggregation process in a variety of neurodegenerative diseases. © 2008 Wiley‐Liss, Inc.