Kinetics of enzyme inhibition by active molluscicidal agents ferulic acid, umbelliferone, eugenol and limonene in the nervous tissue of snail Lymnaea acuminata

Abstract Ferulic acid, umbelliferone ( Ferula asafoetida ), eugenol ( Syzygium aromaticum ) and limonene ( Carum carvi ) are active molluscicidal components that inhibited the activity of alkaline phosphatase and acetylcholinesterase in in vivo and in vitro exposure of Lymnaea acuminata . It was observed that ferulic acid, umbelliferone and eugenol are competitive and limonene is a competitive–non‐competitive inhibitor of alkaline phosphatase. Ferulic acid and umbelliferone are competitive, whereas eugenol and limonene are competitive–non‐competitive and uncompetitive inhibitors of acetylcholinesterase, respectively. Copyright © 2008 John Wiley & Sons, Ltd.