化学
色谱法
串联质谱法
电喷雾
质谱法
检出限
液相色谱-质谱法
串联
柱色谱法
乙腈
材料科学
复合材料
作者
Leen Mortier,Els Daeseleire,Anne-Catherine Huet,Philippe Delahaut,Carlos Van Peteghem
摘要
A sensitive and very selective high-performance liquid chromatography/tandem mass spectrometric (LC/MS/MS) method for the detection of halofuginone in whole egg has been developed. After deproteinisation with acetonitrile and evaporation of the organic solvent, halofuginone was further isolated by applying immunoaffinity chromatography. The concentrated eluent was injected into the LC/MS/MS system on a C18 column. The precursor ion ([M+H]+) produced by positive electrospray ionisation was selected for fragmentation with argon. Validation parameters such as recovery, linearity and repeatability, decision limit (CCα) and detection capability (CCβ) were determined. Copyright © 2004 John Wiley & Sons, Ltd.
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