内科学
链脲佐菌素
内分泌学
医学
牙槽
甲状旁腺激素
骨保护素
骨重建
生理盐水
牙周炎
结扎
骨质疏松症
糖尿病
受体
激活剂(遗传学)
钙
牙科
作者
Helin Chen,Taozi Fu,Yuanyuan Ma,Xiangnan Wu,Xianxian Li,Xinyi Li,Jiefei Shen,Hang Wang
标识
DOI:10.1016/j.archoralbio.2017.06.033
摘要
Intermittent administration of parathyroid hormone (PTH) has been demonstrated to have anabolic effects on bone metabolism and is approved for use in the treatment of osteoporosis. This study evaluates the role of intermittent PTH administration on alveolar bone loss in streptozotocin (STZ)-induced diabetic rats. Fifty male Sprague Dawley rats were randomly divided into the following five groups: (1) a control group (saline placebo without ligature and STZ injection), (2) a PTH group (PTH administration without ligature and STZ injection), (3) an L group (saline placebo with ligature), (4) an L + STZ group (saline placebo with ligature and STZ injection), and (5) an L + STZ + PTH group (PTH administration with ligature and STZ injection). PTH was administered at 75 μg/kg per dose four times a week for 28 days. Subsequently, all rats were sacrificed, and their mandibles were extracted for micro-computed tomography (micro-CT) scanning, as well as histological and immunochemical evaluation. Micro-CT scanning demonstrated the anabolic effect of PTH on alveolar bone metabolism in STZ-induced diabetic rats (P < 0.05), and histomorphometry indicated that PTH inhibited inflammation of the periodontium and increased the level of osteoblastic activity (P < 0.05). Immunochemical evaluation showed that rats subjected to both ligature placement and STZ injection had the highest receptor activator of nuclear factor kappa B ligand (RANKL)/osteoprotegerin (OPG) ratio and that PTH administration decreased this ratio. Intermittent systemic PTH administration effectively reduced alveolar bone loss and ameliorated the manifestation of experimental periodontitis in STZ-induced diabetic rats.
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