副溶血性弧菌
微生物学
哈维氏弧菌
抗菌肽
生物
鳗弧菌
细菌
弧菌
溶藻弧菌
革兰氏阴性菌
抗菌剂
大肠杆菌
生物化学
遗传学
基因
作者
Wei Zhang,Xiaohang Xu,Jun Zhang,Ting Ye,Qiao Zhou,Ying Xü,Wenyi Li,Zhangli Hu,Chenjing Shang
出处
期刊:Pharmaceutics
[MDPI AG]
日期:2022-02-14
卷期号:14 (2): 413-413
被引量:5
标识
DOI:10.3390/pharmaceutics14020413
摘要
Crustins are an antimicrobial peptide (AMP) family that plays an important role in innate immunity in crustaceans. It is important to discover new AMPs from natural sources to expand the current database. Here, we identified and characterized a new crustin family member, named AaCrus1, from Amphibalanus amphitrite. AaCrus1 shares high identity (48.10%) with PvCrus, a Type I crustin of Penaeus vannamei that possesses a whey acidic protein (WAP) domain. AaCrus1 contains 237 amino acids and eight cysteine residues forming conserved 'four-disulfide core' structure. Our recombinant AaCrus1 (rAaCrus 1) could inhibit the growth of two Gram-positive bacteria (Staphylococcus aureus, Bacillus sp. T2) and four Gram-negative bacteria (Vibrio parahaemolyticus, Vibrio harveyi, Vibrio anguillarum, Vibrio alginolyticus) with a minimum inhibitory concentration of 3.5-28 μM. It can further induce agglutination of both Gram-positive and Gram-negative bacteria. rAaCrus1 can bind to bacteria and damage bacterial cell membranes. Furthermore, rAaCrus1 disrupted biofilm development of S. aureus and V. parahaemolyticus. Our discovery and characterization of this new crustin can be further optimized as a good alternative to antibiotics.
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