CRISPR-microfluidic array for single-copy DNA mini barcoding and rapid field species identification

DNA barcode identification has wide applications in taxonomy, molecular phylogenetics, biodiversity science and species identification. This work developed a CRISPR-microfluidic array for rapid identification of DNA barcodes. This assay has successfully distinguish various types of Datura L. with single-copy sensitivity and rapidity. The detection system achieved 100% consistency with the morphological identification in detecting real-field seeds, holding great potential in the field of DNA barcoding and species recognition.