环介导等温扩增
清脆的
回文
严重急性呼吸综合征冠状病毒2型(SARS-CoV-2)
病毒学
生物
基因
临床诊断
脱氧核酶
2019年冠状病毒病(COVID-19)
Cas9
计算生物学
DNA
遗传学
医学
疾病
传染病(医学专业)
病理
临床心理学
作者
Jayeon Song,Baekdong Cha,Jeong Moon,Hyowon Jang,Sun‐Joo Kim,Jieun Jang,Dongeun Yong,Hyung Jun Kwon,In-Chul Lee,Eun‐Kyung Lim,Juyeon Jung,Hyun Gyu Park,Taejoon Kang
出处
期刊:ACS Nano
[American Chemical Society]
日期:2022-06-23
卷期号:16 (7): 11300-11314
被引量:50
标识
DOI:10.1021/acsnano.2c04840
摘要
Coronavirus disease (COVID-19) has affected people for over two years. Moreover, the emergence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) variants has raised concerns regarding its accurate diagnosis. Here, we report a colorimetric DNAzyme reaction triggered by loop-mediated isothermal amplification (LAMP) with clustered regularly interspaced short palindromic repeats (CRISPR), referred to as DAMPR assay for detecting SARS-CoV-2 and variants genes with attomolar sensitivity within an hour. The CRISPR-associated protein 9 (Cas9) system eliminated false-positive signals of LAMP products, improving the accuracy of DAMPR assay. Further, we fabricated a portable DAMPR assay system using a three-dimensional printing technique and developed a machine learning (ML)-based smartphone application to routinely check diagnostic results of SARS-CoV-2 and variants. Among blind tests of 136 clinical samples, the proposed system successfully diagnosed COVID-19 patients with a clinical sensitivity and specificity of 100% each. More importantly, the D614G (variant-common), T478K (delta-specific), and A67V (omicron-specific) mutations of the SARS-CoV-2 S gene were detected selectively, enabling the diagnosis of 70 SARS-CoV-2 delta or omicron variant patients. The DAMPR assay system is expected to be employed for on-site, rapid, accurate detection of SARS-CoV-2 and its variants gene and employed in the diagnosis of various infectious diseases.
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