Toxicological Safety Assessment of Heat‐Treated Lactiplantibacillus plantarum OLL2712 as a Food Ingredient

Lactiplantibacillus plantarum is a Gram-positive, bacilli-shaped bacterium commonly found in fermented foods and the human gastrointestinal tract. Heat-treatment of L. plantarum OLL2712 prior to consumption has been found to have positive health effects. However, a full toxicological evaluation of heat-treated L. plantarum OLL2712 cells (heat-treated OLL2712) has not been conducted. In this regard, heat-treated OLL2712 was evaluated for use as an ingredient in food and dietary supplements using a panel of toxicological tests including genotoxic and subchronic studies. Heat-treated OLL2712 did not show any genotoxicity based on an in vitro mammalian chromosomal aberration test and in vivo mouse micronucleus assay up to 8.0 × 10