Cell line sourcing and characterization for cultured meat product development

This chapter explores the use of bioreactors for cell multiplication in cultured meat product development. The chapter begins by first providing an overview of the principles and structure of a bioreactor, which is followed by a breakdown of bioreactor operation modes: batch cultivation, fed-batch cultivation and continuous cultivation. The chapter also highlights the important bioprocess parameters that need to be considered when creating a stem cell niche in a bioreactor, such as temperature, oxygen, pH level, lactic acid and culture mixing. The chapter considers cultivation anchorage-dependent cells in stirred tank bioreactors, the scale-up of a bioprocess in bioreactors and also highlights areas which could help reduce cost and optimise processing within bioreactors.