羧酸酯酶
化学发光
化学
癌症
细胞内
酶
基因亚型
癌细胞
分子生物学
生物
生物化学
有机化学
基因
遗传学
作者
Cagri Ozsan,Karishma Kailass,Elyse M Digby,Toghrul Almammadov,Andrew A. Beharry,Safacan Kolemen
摘要
Carboxylesterase 2 (CES2) has crucial roles in both xenobiotic metabolism and formation of pathogenic states including cancer. Thus, it is highly critical to monitor intracellular CES2 activity in living cancer cells. Here, we report a CES2 activatable phenoxy 1,2-dioxetane based chemiluminescent agent (CL-CES2). The probe exhibited a selective turn-on response in the presence of CES2 enzyme and enabled detection of CES2 activity in three different cancer cells that possess varying enzyme concentrations with high signal to noise ratios. In contrast no signal was obtained with CES1, an isoform of CES2 enzyme. CL-CES2 marks the first ever example of a CES2-responsive chemiluminescent luminophore and holds a great potential in further understanding the roles of CES2 activity in tumorogenesis.
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