Prior interaction of protein and lipid affects the formation of ternary complexes with starch

The effects of prior interaction between β-lactoglobulin (βLG) and lauric acid (LA) on their formation of ternary complexes with wheat starch (WS) was studied. Firstly, the interaction between βLG and LA after they were heated at different temperatures between 55 and 95 °C was characterized by fluorescence spectroscopy and molecular dynamics simulation. This showed that a greater degree of βLG-LA interaction occurred after heating at higher temperatures. The WS-LA-βLG complexes formed subsequently were analyzed by differential scanning calorimetry, X-ray diffraction, Raman and FTIR spectroscopy, which showed that as interaction of βLG and LA increased there was an inhibitory action on the formation of ternary complex with WS. Hence, we conclude that there is competition in the ternary systems between the protein and starch to interact with the lipid, and that stronger interaction of the protein and lipid may hinder the formation of ternary complexes with starch.