Immune cell‐intrinsic Ah receptor facilitates the expression of antimicrobial REG3G in the small intestine

Abstract The intestinal epithelial layer is susceptible to damage by chemical, physiological and mechanical stress. While it is essential to maintain the integrity of epithelium, the biochemical pathways that contribute to the barrier function have not been completely investigated. Here we demonstrate an aryl hydrocarbon receptor (AHR)‐dependent mechanism facilitating the production of the antimicrobial peptide AMP regenerating islet‐derived protein 3 gamma (REG3G), which is essential for intestinal homeostasis. Genetic ablation of AHR in mice impairs pSTAT3‐mediated REG3G expression and increases bacterial numbers of Segmented filamentous bacteria ( SFB) and Akkermansia muciniphila in the small intestine. Studies with tissue‐specific conditional knockout mice revealed that the presence of AHR in the epithelial cells of the small intestine is not required for the production of REG3G through the phosphorylated STAT3‐mediated pathway. However, immune‐cell‐specific AHR activity is necessary for normal expression of REG3G in all regions of the small intestine. A diet rich in broccoli, capable of inducing AHR activity, increases REG3G production when compared to a semi‐purified diet that is devoid of ligands that can potentially activate the AHR, thus highlighting the importance of AHR in antimicrobial function. Overall, these data suggest that homeostatic antimicrobial REG3G production is increased by an AHR pathway intrinsic to the immune cells in the small intestine.