色谱法
化学
甲酸
分析物
高效液相色谱法
电喷雾电离
串联质谱法
质谱法
药代动力学
选择性反应监测
甲醇
洗脱
电喷雾
液相色谱-质谱法
分析化学(期刊)
医学
有机化学
内科学
作者
Fan Chen,Ziyue Wang,Lvqi Luo,Yifan He,Yizhe Ma,Congcong Wen,Xianqin Wang,Xiuwei Shen
摘要
In this paper, an ultra-high-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method was developed for quantifying the levels of crassicauline A, fuziline, karacoline, and songorine in rat plasma. After processing the rat plasma, the proteins in the plasma were separated by extracting the analytes with acetonitrile-methanol (9:1, v/v). The chromatographic column used was the UPLC HSS T3 column, and the mobile phase (methanol-water with 0.1% formic acid) under a gradient elution profile was used to separate the four compounds, with elution times for each analyte being less than 5 min. Electrospray ionization in positive-ion mode and operating in multiple reaction monitoring mode was used for quantitative analysis. Crassicauline A, fuziline, karacoline, and songorine were administered to 48 rats (n = 6 per group) orally (5 mg/kg) and intravenously (0.5 mg/kg). The standard curves demonstrated excellent linearity in the range of 1-2500 ng/mL, wherein all r values were greater than 0.99. The UPLC-MS/MS method for the determination of crassicauline A, fuziline, karacoline, and songorine in rat plasma was successfully applied in determining their pharmacokinetics parameters, from which their oral bioavailabilities were calculated to be 18.7%, 4.3%, 6.0%, and 8.4%, respectively.
科研通智能强力驱动
Strongly Powered by AbleSci AI