化学
细胞毒性T细胞
萜类
立体化学
生物化学
体外
作者
Wenbai Zhou,Jiao-Yang Hou,Qian Li,Yujue Wang,Jiayi Wang,Ming-Hao Jiang,Guo-Dong Yao,Xiao‐Xiao Huang,Shao‐Jiang Song
出处
期刊:Phytochemistry
[Elsevier]
日期:2022-12-01
卷期号:204: 113468-113468
被引量:6
标识
DOI:10.1016/j.phytochem.2022.113468
摘要
Investigation of the whole plant of Daphne gemmata E. Pritz. ex Diels (Thymelaeaceae) using molecular networking coupled to Network Annotation Propagation (NAP) and unsupervised substructure annotation (MS2LDA) led to the discovery of five tigliane diterpenoids, 14 guaiane sesquiterpenoids, one rhamnofolane diterpenoid and three carotene sesquiterpenoids. The structures of the eight undescribed compounds, daphnorbol A and daphnegemmatoids A-G, were characterized by detailed spectroscopic analyses, NMR and ECD calculations, application of Snatzke's method and single-crystal X-ray diffraction analysis. All isolated compounds were evaluated for their cytotoxic activities against HepG2, A549, and MCF-7 cells by MTT assay. Daphnorbol A exhibited significant cytotoxic activity against HepG2 and A549 cells with IC50 values of 4.06 μM and 6.35 μM, respectively. Prostratin showed potent cytotoxic activity against HepG2 and A549 cells with IC50 values of 6.06 μM and 5.45 μM, respectively. Further Hoechst 33,258 and AO-EB staining assays indicated that daphnorbol A and prostratin could induce apoptosis in HepG2 and A549 cells.
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