成纤维细胞
肺纤维化
上皮-间质转换
癌症研究
特发性肺纤维化
纤维化
气体6
受体
成纤维细胞活化蛋白
生物
肺
信号转导
化学
细胞生物学
医学
病理
内科学
下调和上调
细胞培养
癌症
受体酪氨酸激酶
生物化学
遗传学
基因
作者
Yeji Lee,Minsuk Kim,Hee-Sun Kim,Jihee Lee Kang
标识
DOI:10.1007/s10565-024-09858-5
摘要
Abstract The epithelial-mesenchymal transition (EMT) and fibroblast activation are major events in idiopathic pulmonary fibrosis pathogenesis. Here, we investigated whether growth arrest-specific protein 6 (Gas6) plays a protective role in lung fibrosis via suppression of the EMT and fibroblast activation. rGas6 administration inhibited the EMT in isolated mouse ATII cells 14 days post-BLM treatment based on morphologic cellular alterations, changes in mRNA and protein expression profiles of EMT markers, and induction of EMT-activating transcription factors. BLM-induced increases in gene expression of fibroblast activation-related markers and the invasive capacity of primary lung fibroblasts in primary lung fibroblasts were reversed by rGas6 administration. Furthermore, the hydroxyproline content and collagen accumulation in interstitial areas with damaged alveolar structures in lung tissue were reduced by rGas6 administration. Targeting Gas6/Axl signaling events with specific inhibitors of Axl (BGB324), COX-2 (NS-398), EP1/EP2 receptor (AH-6809), or PGD2 DP2 receptor (BAY-u3405) reversed the inhibitory effects of rGas6 on EMT and fibroblast activation. Finally, we confirmed the antifibrotic effects of Gas6 using Gas6 −/− mice. Therefore, Gas6/Axl signaling events play a potential role in inhibition of EMT process and fibroblast activation via COX-2-derived PGE 2 and PGD 2 production, ultimately preventing the development of pulmonary fibrosis.
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