鼠李糖乳杆菌
MTT法
细胞凋亡
细胞毒性
细胞培养
生物
分子生物学
微生物学
癌细胞
细胞毒性T细胞
细胞外
乳酸菌
化学
体外
癌症
细菌
生物化学
遗传学
作者
Elham Behzadi,Hamideh Mahmoodzadeh Hosseini,Abbas Ali Imani Fooladi
标识
DOI:10.1016/j.micpath.2017.06.016
摘要
Bacterial extracellular vesicles (EVs) have come forth into notice as possible important agent to mediate host-pathogen interactions. In this scientific research, the authors have tried to find out the effect of EVs derived from Lactobacillus rhamnosus GG (LDEVs) on the apoptosis induction in HepG2 cell line. The EVs were purified from the conditioned medium of Lactobacillus rhamnosus GG using ultrafiltration and confirmed by transmission electron microscopy (TEM). The HepG2 cells were treated with different concentrations of purified LDEVs and the cytotoxicity and their effects on the expression of bcl-2 and bax genes were assessed by the MTT assay and semi-quantitative RT-PCR, respectively. The MTT assay showed that only 100 μg/ml of LDEVs had a significant cytotoxic effect on cancer cells (p < 0.05). The apoptotic index (bax/bcl2 expression ratio) was significantly increased after treating with 50 and 100 μg/ml LDEVs (p < 0.05). Increased bax/bcl-2 ratio was led to cancer cell death.
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