生物
拟杆菌
聚糖
细菌
膳食纤维
竞赛(生物学)
纤维
肠道菌群
计算生物学
微生物学
生物化学
生物技术
食品科学
遗传学
生态学
化学
有机化学
糖蛋白
作者
Michael L. Patnode,Zachary Beller,Nathan D. Han,Jiye Cheng,Samantha L. Peters,Nicolas Terrapon,Bernard Henrissat,Sophie Le Gall,Luc Saulnier,David K. Hayashi,Alexandra Meynier,Sophie Vinoy,Richard J. Giannone,Robert L. Hettich,Jeffrey I. Gordon
出处
期刊:Cell
[Elsevier]
日期:2019-09-01
卷期号:179 (1): 59-73.e13
被引量:209
标识
DOI:10.1016/j.cell.2019.08.011
摘要
Development of microbiota-directed foods (MDFs) that selectively increase the abundance of beneficial human gut microbes, and their expressed functions, requires knowledge of both the bioactive components of MDFs and the mechanisms underlying microbe-microbe interactions. Here, gnotobiotic mice were colonized with a defined consortium of human-gut-derived bacterial strains and fed different combinations of 34 food-grade fibers added to a representative low-fiber diet consumed in the United States. Bioactive carbohydrates in fiber preparations targeting particular Bacteroides species were identified using community-wide quantitative proteomic analyses of bacterial gene expression coupled with forward genetic screens. Deliberate manipulation of community membership combined with administration of retrievable artificial food particles, consisting of paramagnetic microscopic beads coated with dietary polysaccharides, disclosed the contributions of targeted species to fiber degradation. Our approach, including the use of bead-based biosensors, defines nutrient-harvesting strategies that underlie, as well as alleviate, competition between Bacteroides and control the selectivity of MDF components.
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