摘要
Journal of Food BiochemistryVolume 44, Issue 9 e13347 FULL ARTICLE Bifunctional peptides with antioxidant and angiotensin-converting enzyme inhibitory activity in vitro from egg white hydrolysates Biying Zhang, orcid.org/0000-0002-8454-3744 Jilin Provincial Key Laboratory of Nutrition and Functional Food, Jilin University, Changchun, P.R. China College of Food Science and Engineering, Jilin University, Changchun, P.R. ChinaSearch for more papers by this authorJingbo Liu, Jilin Provincial Key Laboratory of Nutrition and Functional Food, Jilin University, Changchun, P.R. China College of Food Science and Engineering, Jilin University, Changchun, P.R. ChinaSearch for more papers by this authorChang Liu, Jilin Provincial Key Laboratory of Nutrition and Functional Food, Jilin University, Changchun, P.R. China College of Food Science and Engineering, Jilin University, Changchun, P.R. ChinaSearch for more papers by this authorBoqun Liu, orcid.org/0000-0003-2777-4842 Jilin Provincial Key Laboratory of Nutrition and Functional Food, Jilin University, Changchun, P.R. China College of Food Science and Engineering, Jilin University, Changchun, P.R. ChinaSearch for more papers by this authorYiding Yu, Jilin Provincial Key Laboratory of Nutrition and Functional Food, Jilin University, Changchun, P.R. China College of Food Science and Engineering, Jilin University, Changchun, P.R. ChinaSearch for more papers by this authorTing Zhang, Corresponding Author tingzhang413@hotmail.com orcid.org/0000-0003-4229-6332 Jilin Provincial Key Laboratory of Nutrition and Functional Food, Jilin University, Changchun, P.R. China College of Food Science and Engineering, Jilin University, Changchun, P.R. China Correspondence Ting Zhang, Key Laboratory of Nutrition and Functional Food of Jilin Province, Jilin University, NO. 5333 Xi'an Road, Changchun 130062, P.R. China. Email: tingzhang413@hotmail.comSearch for more papers by this author Biying Zhang, orcid.org/0000-0002-8454-3744 Jilin Provincial Key Laboratory of Nutrition and Functional Food, Jilin University, Changchun, P.R. China College of Food Science and Engineering, Jilin University, Changchun, P.R. ChinaSearch for more papers by this authorJingbo Liu, Jilin Provincial Key Laboratory of Nutrition and Functional Food, Jilin University, Changchun, P.R. China College of Food Science and Engineering, Jilin University, Changchun, P.R. ChinaSearch for more papers by this authorChang Liu, Jilin Provincial Key Laboratory of Nutrition and Functional Food, Jilin University, Changchun, P.R. China College of Food Science and Engineering, Jilin University, Changchun, P.R. ChinaSearch for more papers by this authorBoqun Liu, orcid.org/0000-0003-2777-4842 Jilin Provincial Key Laboratory of Nutrition and Functional Food, Jilin University, Changchun, P.R. China College of Food Science and Engineering, Jilin University, Changchun, P.R. ChinaSearch for more papers by this authorYiding Yu, Jilin Provincial Key Laboratory of Nutrition and Functional Food, Jilin University, Changchun, P.R. China College of Food Science and Engineering, Jilin University, Changchun, P.R. ChinaSearch for more papers by this authorTing Zhang, Corresponding Author tingzhang413@hotmail.com orcid.org/0000-0003-4229-6332 Jilin Provincial Key Laboratory of Nutrition and Functional Food, Jilin University, Changchun, P.R. China College of Food Science and Engineering, Jilin University, Changchun, P.R. China Correspondence Ting Zhang, Key Laboratory of Nutrition and Functional Food of Jilin Province, Jilin University, NO. 5333 Xi'an Road, Changchun 130062, P.R. China. Email: tingzhang413@hotmail.comSearch for more papers by this author First published: 29 June 2020 https://doi.org/10.1111/jfbc.13347Citations: 3Read the full textAboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onEmailFacebookTwitterLinked InRedditWechat Abstract Oxidative stress is frequently associated with hypertension. However, the studies on antihypertensive peptides usually focus on ACE inhibitory activity and ignore antioxidant activity of bioactive peptides. In our research, we optimized enzymatic hydrolysis condition of egg white protein. After that we evaluated both the antioxidant and the ACE-inhibitory activity in vitro of egg white hydrolysates and its purified fractions. Four peptides, LAPYK, SVIRW, PKSVIRW, and ADWAK, which can match to ovotransferrin were identified from fraction with the highest bioactivity. Among them, LAPYK possessed the strongest activity with ABTS scavenging IC50 of 5.29 ± 0.24 µM, ORAC of 1.50 ± 0.04 µM TE/µM, ACE-inhibitory IC50 of 12.65 ± 1.34 µM. For further, we explored inhibitory mechanism of LAPYK against ACE. Research on bifunctional peptides is helpful for the application of antihypertensive peptides. Practical applications At present, the therapeutic drugs for hypertension usually focus on a single target, but the pathogenesis of hypertension is multifactorial, and the combined treatment of multiple targets may have a better therapeutic effect. Compared with commercially available drugs, food-derived bioactive peptides have lower side effects, as well as exhibit multi-bioactivities more easily. Exploration of bifunctional peptides with both ACE inhibitory and antioxidant activity provides new ideas for the treatment of hypertension. Citing Literature Supporting Information Filename Description jfbc13347-sup-0001-FigS1.tifTIFF image, 11.6 KB Fig S1 jfbc13347-sup-0002-TableS1.docxWord document, 14.7 KB Table S1 jfbc13347-sup-0003-TableS2.docxWord document, 16.2 KB Table S2 jfbc13347-sup-0004-TableS3.xlsxapplication/excel, 12.4 KB Table S3 Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article. Volume44, Issue9September 2020e13347 RelatedInformation