异型生物质的
生物
单加氧酶
细胞色素P450
异源表达
药物代谢
转化(遗传学)
计算生物学
酶
生物化学
基因
重组DNA
作者
Ralf Nauen,Christoph Zimmer,John Vontas
标识
DOI:10.1016/j.cois.2020.10.011
摘要
Insect cytochrome P450-monooxygenases (P450s) are an enzyme superfamily involved in the oxidative transformation of endogenous and exogenous substrates, including insecticides. They were also shown to determine insecticide selectivity in beneficial arthropods such as bee pollinators, and to detoxify plant secondary metabolites. The recent explosion in numbers of P450s due to increased invertebrate genomes sequenced, allowed researchers to study their functional relevance for xenobiotic metabolism by recombinant expression using different expression systems. Troubleshooting strategies, including different systems and protein modifications typically adapted from mammalian P450s, have been applied to improve the functional expression, with partial success. The aim of this mini review is to critically summarize different strategies recently developed and used to produce recombinant insect P450s for xenobiotic metabolism studies.
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