Morphological changes in the experimental model of polycystic ovary syndrome and effects of vitamin D treatment

Abstract Aim This research was designed to develop an animal model by inducing polycystic ovary syndrome (PCOS) with dehydroepiandrosterone (DHEA) and observe reproductive and morphological changes after treatment with vitamin D. Materials and Methods Thirty pre‐pubertal female Sprague–Dawley (SD) dams were recruited. The animals were distributed 10 each in control, PCOS and vitamin D‐treated groups. In control group 0.2 ml of sesame oil was given. PCOS group was administered DHEA by the daily dose of 6 mg/kg for 30 days. In vitamin D‐treated group, animals were injected 6 mg/kg/day DHEA daily and 120 ng 1, 25(OH) 2D3/100 g subcutaneously once a week. The occurrence of reproductive phenotypic PCOS was evaluated by estrous cycle, morphology and histological changes of ovary, uterus on light microscope. Results The results of this study showed significant weight gain, obesity, and estrous irregularity in PCOs group as compared to control and vitamin D‐treated group. Conclusion Administration of vitamin D (120 ng 1, 25(OH) 2D3/100) improved the cycle characteristics, reduced body weight and morphological features in PCOS induced animals. The results support the effect of vitamin D treatment for metabolic and reproductive characteristic features in PCOS females.