Expression and clinical significance of circular RNAs related to immunity and inflammation in patients with rheumatoid arthritis

The aim of this study was to investigate the changes in and clinical significance of circular RNAs (circRNAs) expression related to immunity and inflammation in patients with rheumatoid arthritis (RA). We analyzed the circRNAs related to immunity and inflammation in peripheral blood mononuclear cells (PBMCs) from three RA patients and three healthy controls using high-throughput sequencing and bioinformatics analysis. Subsequently, four differentially expressed circRNAs were verified by reverse transcriptase quantitative polymerase chain reaction (RT-qPCR). We selected hsa_circ_0003353 for functional phenotype analysis in fibroblast-like synoviocytes (FLS) according to correlation tests and logistic regression analysis. The results identified and verified the expression of circRNAs (0003353, 0005732, 0072428, 0091685) that were significantly changed in PBMCs from RA patients. ROC analysis suggested that these circRNAs have significant value in RA diagnosis. Spearman analysis revealed that significant relationships between these circRNAs and clinical indicators as well as SPP. The logistic regression analysis showed that clinical indicators and SPP were risk factors for hsa_circ_0003353 expression, which is worthy of further investigation. As predicted, hsa_circ_0003353 had significantly higher expression in RA-FLS. The cell proliferation, migration, invasion, apoptosis, and inflammatory cytokine production of RA-FLS showed significant alterations after hsa_circ_0003353 suppression and overexpression. Our results indicate that hsa_circ_0003353 may play an essential role in promoting immunity, inflammation, synovial invasion, and joint destruction and that hsa_circ_0003353 may act as a biomarker of RA.