抗氧化剂
异黄酮
化学
超氧化物歧化酶
谷胱甘肽过氧化物酶
生物化学
谷胱甘肽
脂质过氧化
芒柄花素
发酵
体内
生物转化
天冬氨酸转氨酶
生物
酶
类黄酮
碱性磷酸酶
大豆黄酮
染料木素
生物技术
内分泌学
作者
Ji Yeon Lee,Hye Min Park,Chang-Ho Kang
出处
期刊:Fermentation
[Multidisciplinary Digital Publishing Institute]
日期:2022-01-16
卷期号:8 (1): 34-34
被引量:7
标识
DOI:10.3390/fermentation8010034
摘要
In this study, the antioxidant mechanism of Astragalus membranaceus fermented by Lactiplantibacillusplantarum MG5276 (MG5276F-AM) was evaluated in HepG2 cells and in an animal model. HPLC analysis was performed to confirm the bioconversion of the bioactive compounds in A. membranaceus by fermentation. Calycosin and formononetin, which were not detected before fermentation (NF-AM), were detected after fermentation (MG5276F-AM), and its glycoside was not observed in MG5276F-AM. In HepG2 cells, MG5276F-AM alleviated H2O2-induced oxidative stress by mediating lipid peroxidation and glutathione levels, and upregulated antioxidant enzymes including catalase (CAT), superoxide dismutase (SOD), and glutathione peroxidase (GPx). In the tBHP-injected mouse model, administration of MG5276F-AM reduced hepatic aspartate transaminase, alanine transaminase, and lipid peroxidation. MG5276F-AM also modulated antioxidant enzymes as well as HepG2 cells. Thus, fermentation of A. membranaceus with L. plantarum MG5276 elevated the isoflavonoid aglycone by hydrolysis of its glycosides, and this bioconversion enhanced antioxidant activity both in vitro and in vivo.
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