Phytic Acid Maintains the Integrity of the Blood–Milk Barrier by Regulating Inflammatory Response and Intestinal Flora Structure

The destruction of the blood-milk barrier (BMB) caused by the mammary inflammatory response (MIR) is one of the main reasons that hinders breastfeeding. To relieve the inflammatory response and maintain BMB, we found that phytic acid (PA) has good anti-inflammatory activity. Therefore, we focused on researching the influence and mechanism of PA on BMB and MIR. We constructed a mammary inflammatory response model using lipopolysaccharide (LPS) in vivo, and we used mammary epithelial cells (mMECs) to construct a cell inflammatory response model in vitro. The results showed that PA alleviated mammary tissue damage and reduced the production of inflammatory mediators (such as IL-1β and iNOS) in mammary tissue and mMECs. PA also maintained the integrity of the BMB in mice by increasing the expression of tight junction proteins. 16S rDNA high-throughput sequencing showed that PA significantly ameliorated the intestinal flora of model mice. Mechanism studies showed that PA exerted an anti-MIR effect by inhibiting the AKT/NF-κB signaling pathway. In summary, our study found that PA maintains the integrity of BMB by regulating the inflammatory response and intestinal flora structure.