SNP Molecular Markers Development and Genetic Diversity Analysis of <i>Forsythia suspensa</i> Based on SLAF-seq Technology

Forsythia suspensa  (Thunb.) Vahl is an important medicinal plant that has great value to study in China. In this study, the 39  Forsythia suspensa  materials were used specific loci amplified fragment sequencing technology (SLAF-seq) to develop SNP molecular markers. A total of 112.28 Mb reads data were obtained by sequencing. The reads data of each sample ranged from 1 324 860~5 911 565. The average sequencing quality value (Q30) and GC content of samples was 96.29% and 36.97%, respectively. Analysis of bioinformatics, there were 535 357 SLAF tags, in which 262 297 SLAF tags were polymorphic, and the average sequencing depth of the samples was 16.20 x. A total of 1 809 741 SNP molecular markers were obtained and 39  Forsythia suspensa materials were divided into 4 groups. This study could provide theoretical basis for germplasm resource identification and genetic diversity analysis of  Forsythia suspensa .