量子点
材料科学
石墨烯
检出限
生物传感器
电极
纳米技术
电化学
化学
色谱法
物理化学
作者
Hai Linh Tran,Van Dien Dang,Naresh Kumar Dega,Sih-Min Lu,Yu‐Fen Huang,Ruey‐an Doong
标识
DOI:10.1016/j.snb.2022.132233
摘要
In this study, an ultrasensitive electrochemical biosensor fabricated from the deposition of nitrogen-doped graphene quantum dots (NGQDs) and phytohemagglutinin-L (PHA-L) onto screen-printed electrodes (SE) was successfully developed for breast cancer cell (MCF-7) detection in human serum. NGQDs with particle sizes of 3–12 nm were fabricated via a microwave-assisted hydrothermal method using passion fruit juice as the green carbon source, and then were conjugated with PHA-L through the amide bond between the COOH group of NGQDs and the NH 2 group of PHA-L. The dual-functionalized NGQDs can enhance the electrical conductivity of the electrochemical sensor and serve as the nanocargo for PHA-L, a highly specific receptor for effective anchoring of MCF-7, and subsequently result in the ultrasensitive and highly selective detection of MCF-7. The prepared SE= |NGQDs/PHA-L sensor exhibited a wide linear detection range from 5 to 10 6 cells mL −1 in phosphate-buffered saline (PBS) and 20–10 6 cells mL −1 in human serum with extremely low detection limits of 1 and 2 cells mL −1 , respectively. Moreover, the superior selectivity over 6 different interferents and long-term stability after 80 d of storage in human serum make the NGQDs/PHA-L complex an excellent sensing probe that can electrochemically detect low concentrations of cancer cells for the early diagnosis and treatment of breast cancer and other malignant neoplasms. • An ultrasensitive immunosensor was developed for MCF-7 detection and bioimaging. • The NGQDs enhance the electric conductivity of immunosensor and also act as the nanocargo of PHA-L. • PHA-L is a highly specific receptor for ultra-sensitive and selective detection of MCF-7 cells. • The sensor exhibits a wide linear range of 20–10 6 cells mL −1 in human serum with LOD of 2 cells mL −1 . • A superior selectivity over cancer makers and long-term stability after 80 d of storage is obtained.
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