油尿苷
羟基酪醇
化学
DPPH
酶水解
水解
多酚
抗氧化剂
水解物
食品科学
色谱法
生物化学
作者
Jiaojiao Yuan,Chengzhang Wang,Jianzhong Ye,Ran Tao,Yu-Si Zhang
出处
期刊:Molecules
[MDPI AG]
日期:2015-02-11
卷期号:20 (2): 2903-2921
被引量:72
标识
DOI:10.3390/molecules20022903
摘要
Oleuropein (OE), the main polyphenol in olive leaf extract, is likely to decompose into hydroxytyrosol (HT) and elenolic acid under the action of light, acid, base, high temperature.In the enzymatic process, the content of OE in olive leaf extract and enzyme are key factors that affect the yield of HT.A selective enzyme was screened from among 10 enzymes with a high OE degradation rate.A single factor (pH, temperature, time, enzyme quantity) optimization process and a Box-Behnken design were studied for the enzymatic hydrolysis of 81.04% OE olive leaf extract.Additionally, enzymatic hydrolysis results with different substrates (38.6% and 81.04% OE) were compared and the DPPH antioxidant properties were also evaluated.The result showed that the performance of hydrolysis treatments was best using hemicellulase as a bio-catalyst, and the high purity of OE in olive extract was beneficial to biotransform OE into HT.The optimal enzymatic conditions for achieving a maximal yield of HT content obtained by the regression were as follows: pH 5, temperature 55 °C and enzyme quantity 55 mg.The experimental result was 11.31% ± 0.15%, and the degradation rate of OE was 98.54%.From the present investigation of the antioxidant activity determined by the DPPH method, the phenol content and radical scavenging effect were both decreased after enzymatic hydrolysis by hemicellulase.
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