Comparison of Biochemical Parameters in Eggs and Ovarian Fluid of Amur Sturgeon Acipenser schrenckii, Siberian Sturgeon A.baeri and Hybrid Sturgeon

The biochemical compositions and enzymatic activities in eggs and ovarian fluid were investigated in Amur sturgeon Acipenser schrenckii, Siberian sturgeon A.baeri and hybrid sturgeon(Amur sturgeon ♂ × Siberian huso Huso dauricus ♀)by biochemical methods. The results showed that there was significant larger egg diameter in Amur sturgeon and the hybrid sturgeon than in Siberian sturgeon(P0.050). The hybrid sturgeon had significantly greater diameter in the hydrated and hardening eggs than Amur sturgeon, and Siberian sturgeon(P 0.05). The maximal glutamate-oxaloacetate(GOT, 2.83 U·g-1), acid phosphatase(ACP, 0.69 U·g-1),and alkaline phosphatase(AKP, 0.81 U·mg-1) was observed in the eggs of the hybrid sturgeon, without significant differences among the three sturgeon, except the significantly higher SDH activity in eggs of Amur sturgeon and significantly higher ACP activity in ovarian fluid of the hybrid(P0.05). There were significant differences in composition of protein, trace elements and vitamins and activities of enzymes in egg and ovarian fluid among the three sturgeon species. There was higher Fe content in eggs in Siberian sturgeon than that in Amur sturgeon and the hybrid sturgeon. In ovarian fluid, however, levels of Fe and Zn were found undetectable in Siberian sturgeon. There was significantly lower Vc content in ovarian fluid in Siberian sturgeon than that in Amur sturgeon and the hybrid(P0.05). The significant differences in SDH, and ACP activities and Fe and Vc contents in eggs and ovarian fluid indicate that the differentnutrition should be provided during sturgeon broodstock cultivation.