Expression of Toll-like receptors(TLR)2 and TLR4 in macrophages of peritoneal cavity of mice induced by lipopolysaccharides in vitro

Objective To investigate the effects of increasing stimulating time on expression of TLR2 and TLR4 in peritoneal macrophages of mice ( M induced by LPS in vitro. Methods M were obtained from BALB/C mice in routine. All the cells were divided to six groups, there were 3 repetitions in each group, in which M were incubated with 1靏/ml of LPS(final concentrations) at 37℃ in 5%CO2/95% air for 0, 1.5, 3, 6, 16, 24h respectively and expression of TLR2 and TLR4 were measured by flow cytometry, monoclonal antibodies against TLR2 and TLR4, which were marked by FITC and PE respectively,were used. Results 1.With the stimulating time extending the TLR2 positive percentage went up and the fastigium was at 6h and then it went down slowly. The positive percentage of stimulated groups significantly higher than that of unstimulated group (P0.05). And the MFIs of TLR2 went down with the process of time significantly. 2.With the stimulating time extending the TLR4 positive percentage went up gradually. The positive percentage 24h group was 14.02%±1.23% significantly higher than that of 0h group(8.98%±1.06%, P0.01). The MFI of TLR4 also increased with time extending. The MFIs of stimulated groups were all significantly higher than that of 0h group. Conclusions LPS can induce different expression and distribution of TLR2 and TLR4 on M surface, which indicated that both TLR2 and TLR4 take part in responding actions against LPS.