化学
质谱法
色谱法
等压法
电喷雾
分析化学(期刊)
电喷雾电离
三级四极质谱仪
分辨率(逻辑)
四极
样品制备
串联质谱法
分析物
选择性反应监测
人工智能
物理
热力学
原子物理学
计算机科学
作者
Timon Geib,Lekha Sleno,R Hall,Caroline S. Stokes,Dietrich A. Volmer
标识
DOI:10.1007/s13361-016-1412-2
摘要
We describe a systematic comparison of high and low resolution LC-MS/MS assays for quantification of 25-hydroxyvitamin D3 in human serum. Identical sample preparation, chromatography separations, electrospray ionization sources, precursor ion selection, and ion activation were used; the two assays differed only in the implemented final mass analyzer stage; viz. high resolution quadrupole-quadrupole-time-of-flight (QqTOF) versus low resolution triple quadrupole instruments. The results were assessed against measured concentration levels from a routine clinical chemiluminescence immunoassay. Isobaric interferences prevented the simple use of TOF-MS spectra for extraction of accurate masses and necessitated the application of collision-induced dissociation on the QqTOF platform. The two mass spectrometry assays provided very similar analytical figures of merit, reflecting the lack of relevant isobaric interferences in the MS/MS domain, and were successfully applied to determine the levels of 25-hydroxyvitamin D for patients with chronic liver disease. Graphical Abstract ᅟ.
科研通智能强力驱动
Strongly Powered by AbleSci AI