[Objective]The research aimed to study the effects of 30 kinds of Chinese herbs on bacteria in food. [Method]30 kinds of Chinese herbs and 3 usual bacteria in foods were used for experiment material. And the effects of water,ethanol absolute and petroleum ether extracts on bacteria were studied using the filter paper method. [Result]The results showed that the water extracts of Coptis chinensis Franch. ,Rheum officinale Baill. ,Magnolia officinalis Rehd. et Wils. and Syzygium aromaticum Thunb. ,the ethanol extracts of Coptis chinensis Franch. ,Magnolia officinalis Rehd. et Wils. ,Polygonum cuspidatum Sieb. et zucc. and Syzygium aromaticum Thunb. and the petroleum ether extracts of Allium sativum L. ,Magnolia officinalis Rehd. et Wils. ,Syzygium aromaticum Thunb. and Coptis chinensis Franch. had strength antimicrobial ability. [Conclusion]The study can provide the scientific basis for exploring the natural food preservatives.