Cell free fetal DNA from maternal plasma for β-thalassemia prenatal diagnosis

Objective:To establish a useful cell free fetal DNA(cff-DNA) extracting and detecting method for β-thalassemia prenatal diagnosis.Methods:26 pregnant women were sampled by invasive and noninvasive ways respectively,traditional genetic diagnosis and cff-DNA detecting were followed.Results:Among 26 samples,8 cases were identified as normal,11 and 7 were identified as heterozygous and duplex heterozygous by traditional genetic diagnosis;10 cases were detected as IVS-Ⅱ-654 mutation,15 negatives,1 failure in all 26 cff-DNA tests.Conclusions:Using cff-DNA for prenatal diagnosis is an effective method,But maternal cell free-DNA is an influence fartor in the tests.