The effect of TGF-β_1 to human monocyte-derived dendritic cells

Objective:To investigate the effect of TGF-β_1 on human monocyte-derived Dcs. Methods:Add different combination of cytokines including TGF-β_1, IL-2+TGF-β_1, CD40L+TGF-β_1 and TNF-α+TGF-β_1 when inducing the maturation of DCs, then compare the expression of CD50, CD83, CD80, CD1-α, CD86, HLA-DR and CCR7, the concentration of IL-12 in DCs supernatant and the ability of DCs to prime autologous T cells.Results:The expression levels of CD83, CD80, CD86, HLA-DR and CCR7 and the concentration of IL-12 in TGF-β_1 group are notably decreased(P0.05); the DCs in TNF-α group show stronger ability to activate T cells than other two groups in day 10.Conclusion:TGF-β_1 show impressed negative inhibition to DCs; TNF-α can reverse the inhibition of TGF-β_1 to DCs.