The strategies for efficient expression of recombinant protein in Pichia pastoris

Pichia pastoris expression system is an ideal choice for the production of heterologous proteins,however,not all the heterologous proteins can be successfully and efficiently expressed in the host,there are distinct differences among protein yield levels,bioactivity and stability.The article summarized the main factors impacting the efficient expression of recombinant proteins in P.pastoris,and described the strategy for the efficient expression.