As one of the important of greenhouse gases,the source of nitrous oxide is described,as well as the producing mechanism,the nitrous oxide flux at water-air interface in Taihu Lake is inspected and the daily change characteristics of nitrous oxide flux in the four seasons are analyzed.The results show that the Taihu Lake is the resource of nitrous oxide at most time and the collection at short time.Nitrous oxide fluxes in spring,summer,autumn and winter are 0.018 mg/(m2·h),0.065 mg/(m2·h),0.003 mg/(m2·h) and-0.002 mg/(m2·h) respectively.Nitrous oxide flux in summer is high,but low in other seasons.