Transplanted Ginkgo biloba seedlings were treated with different contents of indoleacetic acid (IAA) solution in 2001. The results indicated that 500 μg/g and 250 μg/g of IAA treatments could significantly increase the height of Ginkgo biloba seedlings, and relatively enhance the diameter and leaf number of Ginkgo biloba seedlings when compared with other IAA treatments and CK. Considering treatment effect and cost, 500 μg/g and 250 μg/g of IAA treatments were the best.